ISSN 0970-7700
 

Original Research 


ANTIVIRAL ACTIVITY OF SELECTED INDIAN MEDICINAL HERBS AGAINST HEPATITIS E VIRUS (HEV) IN THE ESTABLISHED PORCINE CELL MODEL

KISLAY ROY; JAGAT R KANWAR; TOMAZ LANGERHOLC.

Abstract
Background: It is known that hepatitis E virus (HEV) is zoonotically transmitted to humans and while other forms of hepatitis have been in focus for drug and vaccine development, HEV related mortality in case of pregnant women has raised upto 20%. Methods: HEV proteins, were successfully extracted from 22 pig stool samples and the presence of HEV capsid antigen encoded by the open reading frame 2 (ORF2) was confirmed suing dot blot and western blotting in 4 samples. HEV proteins were inoculated in pig epithelial cells (CLAB) and pig and goat macrophage cell lines (PoM2 and GOMA) to develop an in vitro model for observing the cytopathic effects (CPE). The HEV infected cell cultures were subjected to extracts obtained from Kaempfaria galanga (Galangal), Mimosa Pudica (Touch me not), Coleus aromaticus (Doddapatre) and Paederia foetida (Stinkvine). Results: We showed that a single treatment of 56.34 ug/ml HEV protein for 3 hours induced characteristic growth patterns of cells until 4 passages. We also found that extracts of K. galanga significantly (p<0.001) increased cell viability in CLAB cells while other extracts had cytotoxic effects. In the preventive study the both 10-2 and 10-3 dilution of K. galanga extracts significantly (p<0.001) prevented a decrease in cell viability CLAB cells after HEV infection. The 10-2 dilution of P. foetida also showed significant (p<0.001) effects. Extracts of K. galanga and M. Pudica were found to have a significant (p<0.001) preventive effect in PoM2 cells. Whereas, in treatment study, 10-2 dilution of C. aromaticus (p<0.01) and 10-3 dilution of P. foetida (pd 0.001) increased cell viability after HEV infection in CLAB cells. 10-2 dilution of K. galanga was the only effective (p<0.001) extract in case of HEV infected PoM2 cells. Conclusion: We were the first to report CLAB and PoM2 cells as an in vitro model to study the CPE of HEV. We were also the first to report the activity of these medicinal extracts against HEV in a cell culture model. Extracts of K. galanga and P. foetida showed a promising anti-viral activity and the potential to be used as a natural therapy against HEV.

Key words: antiviral, cytopathic, extract, Hepatitis E virus (HEV), Indian Medicinal Herbs, zoonotic,


 
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Pubmed Style

ROY K, KANWAR JR, LANGERHOLC T. ANTIVIRAL ACTIVITY OF SELECTED INDIAN MEDICINAL HERBS AGAINST HEPATITIS E VIRUS (HEV) IN THE ESTABLISHED PORCINE CELL MODEL. J Res Educ Indian Med . 2017; 23(1-2): 3-12. doi:10.5455/JREIM.82-1496389244


Web Style

ROY K, KANWAR JR, LANGERHOLC T. ANTIVIRAL ACTIVITY OF SELECTED INDIAN MEDICINAL HERBS AGAINST HEPATITIS E VIRUS (HEV) IN THE ESTABLISHED PORCINE CELL MODEL. http://www.jreim-ayushjournal.com/?mno=268397 [Access: April 29, 2020]. doi:10.5455/JREIM.82-1496389244


AMA (American Medical Association) Style

ROY K, KANWAR JR, LANGERHOLC T. ANTIVIRAL ACTIVITY OF SELECTED INDIAN MEDICINAL HERBS AGAINST HEPATITIS E VIRUS (HEV) IN THE ESTABLISHED PORCINE CELL MODEL. J Res Educ Indian Med . 2017; 23(1-2): 3-12. doi:10.5455/JREIM.82-1496389244



Vancouver/ICMJE Style

ROY K, KANWAR JR, LANGERHOLC T. ANTIVIRAL ACTIVITY OF SELECTED INDIAN MEDICINAL HERBS AGAINST HEPATITIS E VIRUS (HEV) IN THE ESTABLISHED PORCINE CELL MODEL. J Res Educ Indian Med . (2017), [cited April 29, 2020]; 23(1-2): 3-12. doi:10.5455/JREIM.82-1496389244



Harvard Style

ROY, K., KANWAR, . J. R. & LANGERHOLC, . T. (2017) ANTIVIRAL ACTIVITY OF SELECTED INDIAN MEDICINAL HERBS AGAINST HEPATITIS E VIRUS (HEV) IN THE ESTABLISHED PORCINE CELL MODEL. J Res Educ Indian Med , 23 (1-2), 3-12. doi:10.5455/JREIM.82-1496389244



Turabian Style

ROY, KISLAY, JAGAT R KANWAR, and TOMAZ LANGERHOLC. 2017. ANTIVIRAL ACTIVITY OF SELECTED INDIAN MEDICINAL HERBS AGAINST HEPATITIS E VIRUS (HEV) IN THE ESTABLISHED PORCINE CELL MODEL. Journal of Research and Education in Indian Medicine, 23 (1-2), 3-12. doi:10.5455/JREIM.82-1496389244



Chicago Style

ROY, KISLAY, JAGAT R KANWAR, and TOMAZ LANGERHOLC. "ANTIVIRAL ACTIVITY OF SELECTED INDIAN MEDICINAL HERBS AGAINST HEPATITIS E VIRUS (HEV) IN THE ESTABLISHED PORCINE CELL MODEL." Journal of Research and Education in Indian Medicine 23 (2017), 3-12. doi:10.5455/JREIM.82-1496389244



MLA (The Modern Language Association) Style

ROY, KISLAY, JAGAT R KANWAR, and TOMAZ LANGERHOLC. "ANTIVIRAL ACTIVITY OF SELECTED INDIAN MEDICINAL HERBS AGAINST HEPATITIS E VIRUS (HEV) IN THE ESTABLISHED PORCINE CELL MODEL." Journal of Research and Education in Indian Medicine 23.1-2 (2017), 3-12. Print. doi:10.5455/JREIM.82-1496389244



APA (American Psychological Association) Style

ROY, K., KANWAR, . J. R. & LANGERHOLC, . T. (2017) ANTIVIRAL ACTIVITY OF SELECTED INDIAN MEDICINAL HERBS AGAINST HEPATITIS E VIRUS (HEV) IN THE ESTABLISHED PORCINE CELL MODEL. Journal of Research and Education in Indian Medicine, 23 (1-2), 3-12. doi:10.5455/JREIM.82-1496389244





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